Keywords:
Antiplasmodial activity, Sybr Green assay, acute oral toxicity, field isolates, reference strain
Abstract
Background: Malaria is one of the most important diseases in Republic of Congo with 47.9% of outpatients visit for 64.8% admissions. The rise of antimalarial resistant strains requires the active search of new active compounds and medicinal plants can be an alternative. From an ethnobotanical survey a list of plants with antimalarial reputations was drawn up, including Rauvolfia vomitoria.
Aim: The objective of this study is to see if we can validate the supposed activity of Rauvolfia vomitoria and its toxicity.
Material and Methods: Rauvolfia vomitoria leaves have been collected, dried and sprayed. Leaves powder were macerated in distilled water for 24 hours. Another part of leaves powder were macerated for 24 hours successively in hexane, dichloromethane, mix of diclhoromethane: methanol (v:v) and methanol. The crude extracts were prepared and tested for antiplasmodial activity on NF54 strains and field isolates with the SYBR Green I-based in vitro assay technique. The extracts with the best antiplasmodial activities were used on rats for acute toxicity.
Results: All Rauvolfia vomitoria leaves extracts have shown a very good antiplasmodial activity (0.63 CI50 20.19 µg/ml) and no toxicity up to 2000mg/kg.
Conclusion: Rauvolfia vomitoria leaves have an antiplasmodial activity. This study confirms the use of the plant by the traditional healers. We will pursue the work to find the active compounds of the plant.
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