DOI:
Abstract
Poliovirus (PV), an enterovirus of the Picornaviridae family, is the agent of poliomyelitis , a devastating neurologic disease that may result in paralysis and even death . Mangiferin is the major constituent of Mangifera indica and has multiple pharmacological activities, including antiviral. This study evaluated the activity of mangiferin against poliovirus type-1 (PV-1) . The compound exhibited the 50% cytotoxic concentration (CC 50 ) > 2000 μg/mL in HEp-2 cell cultures, by the dimethylthiazolyl-diphenyltetrazolium bromide method (MTT). The 50% inhibitory concentration (IC50) was of 53.5 μg/mL, determined by the plaque reduction assay (PRA) with selectivity index (SI) > 37.4. We investigate the possible mechanism of action of mangiferin and a maximum inhibition of PV was found for virucidal and inhibition of adsorption assays, at all tested concentrations. The inhibition of viral protein synthesis was also demonstrated by immunofluorescence assay (IFA) . These results suggested that mangiferin is an attractive candidate for the control of PV infection .
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