EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF KYDIA CALYCINA ROXB., AERIAL PARTS USING DPPH AND SUPEROXIDE RADICALS

Authors

K. Manikya Kumari

Add affilation 29
V. Padmaja

Professor,
Department of Botany,
Andhra University, Visakhapatnam-530003, AP,

DOI:

https://doi.org/10.7439/ijpr.v2i4.4217

Abstract

Back ground: Free radicals are chemical species possessing an unpaired electron which are generally very reactive. The role of free radicals has been implicated in the causation of several diseases such as liver cirrhosis, cancer, aging, arthritis, diabetes, etc. and the compounds that can scavenge free radicals have great potential in ameliorating these disease process. Medicinal herbs are considered to be good therapeutics since time immemorial. Kydia calycina Roxb., is a widely used ethnomedicinal plant in the north coastal districts of Andhra Pradesh, India, to treat various liver disorders, skin related problems and other common ailments. Method: The DPPH radical and superoxide radical scavenging activity of hydro-alcoholic extract, hexane, ethyl acetate and methanol fractions of aerial parts of Kydia calycina Roxb., was determined by the IC₅₀ values of the selected plant extracts and ascorbic acid. The lower the IC50 value, the higher the free radical scavenging activity. Result: The mean IC₅₀ values for DPPH radical with 80% hydro-alcoholic extract, hexane fraction, ethyl acetate fraction and methanol fraction of Kydia calycina aerial parts were found to be 73.5, 36.0, 23.0 and 25.9 g. Among the test results, ethyl acetate fraction showed better activity. The mean IC₅₀ values for Superoxide radical with 80% hydro-alcoholic extract, hexane fraction, ethyl acetate fraction and methanol fraction of Kydia calycina aerial parts were found to be 30.2, 21.6, 18.7 and 25.9 g. Conclusion: The test results revealed, the methanol extract in general and ethyl acetate fraction in specific showed better activity. The results were found to be significant, validating the free radical scavenging activity of Kydia calycina .

Downloads

Download data is not yet available.

References

1. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacology and Therapeutics 2002; 96:67-202.
2. Hertog MGL, Kkromhout D, Aravanis C, Blackburn H, Bizina R, Fidanza F . Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch Internal Med. 1995; 115:381-386.
3. Wang M, Li J, Rangarajan M, Shao Y, La Voie EJ, Huang TC, Ho CT. Antioxidative phenolic compounds from sege (Salvia officinalis). J Agric Food Chem.1998; 46:4869-4873.
4. Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci. 2000; 66: 723-35.
5. Jitendra B. Jain, Sheetal C Kumara, Bhattacharya S. Medicinal flora of Madhya Pradesh and Chattisgarh. A review of Indian Journal of Traditional Knowledge. 2006; 5(2): 237-242.
6. Khare CP. India Medicinal Plants, An Ilustrated Dictionary, Springer Reference, Springer 2012.
7. Munesh Kumar, Joshua Judex Lakianga, Gopichand B. Phytotoxic effects of agroforestery tree crops are germination and radical growth of some food crops of Mizorom. Lyonia, A Journal of Ecology and Application 2006; 11(2): 83-89.
8. Braca A. Fico G, Morelli I, De Simone F, Tome F, De Tommasi N. Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J. Ethnopharmacol 2003; 86; 63-67.
9. Mc Cord JM, Fridovich I. Superoxe dismutase: an enzymatic function for erythrocuprien. J Biol Chem. 1969; 244: 6049-55.
10. Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63: 1035-42.
11. Halliwell B, Aeschbach R, Loliger J, Aruoma OI. The characterization of antioxidants. Food Chem Toxicol 1994; 33: 601-617.
12. Dragland S, Senoo H, Wake K, Holte K, Blomhoff R. Several culinary and medical herbs are importants sources of dietary antioxidants. J. Nutrition. 2003; 133:1286-1290.
13. Farine JP, Legal L, Moreteau B, Le Quere JL. Volatile components of ripe fruits of Morinda citrifolia and their effects on Drosophila. Phytochemistry. 1996; 41: 433-8.

Published

2012-12-29

How to Cite

Kumari KM, Padmaja V. . Int J of Pharmc Res [Internet]. 2012Dec.29 [cited 2020Jul.3];2(4):137-42. Available from: https://ssjournals.net/index.php/ijpr/article/view/4217

Issue

Vol. 2 No. 4 (2012): Oct – Dec

Section

Research Articles

Authors who publish with this journal agree to the following terms:

1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
4. Author must Submit Copyright form After acceptance of Article