DOI:
https://doi.org/10.7439/ijpr.v6i6.3311
Abstract
The aim of the present study was to evaluate the immunogenicity of a generic product of erythropoietin registered in Jordan, by detecting the presence of anti-recombinant human erythropoietin antibodies in the serum via an ELISA technique. Materials and Methods : Briefly, polystyrene micro-titer plates (96-well) were coated with rhEPO (the generic product) at 10 g/1mL. Goat anti-human IgG:HRP or rabbit polyclonal to human IgM:HRP was added to the wells and incubated. A prepared substrate solution was then added to each well. The absorbance was measured with a microplate reader after green color development (n=3). The sera of 95 patients were tested for the presence of IgM or IgG antibodies. Results : Antibodies were detected in 26.3% of the population; where 16.8% were found to have only IgG antibodies, 7.4% had only IgM antibodies, and 2.1% had both antibodies. Cigarette smoking correlated significantly with the development of IgG antibodies. Moreover folate administration correlated inversely with decreasing the risk of developing IgM antibodies . Conclusions : this study proves the immunogenic effect for this product
Downloads
- PDF
Published
How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
-
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- Author must Submit Copyright form After acceptance of Article