Immunomodulation Potential of Andrographis Paniculata and Tinospora Cordifolia Methanolic Extracts in Combination Forms



  • Rajesh Yadav

    Department of Pharmacy,
    SRMS, College of Engg.and Tech., Bareilly, U.P.

  • N Yadav

    Department of Pharmacy,
    SRMS, College of Engg.and Tech., Bareilly, U.P.

  • M D Kharya

    Department of Pharmaceutical Sciences,
    Dr. H. S. Gour Central University, Sagar, M.P.



Pregnancy induced hypertension, maternal outcome, perinatal outcome


Objective: This work mainly focuses on the immunomodulatory effect of different drug combinations (DC-I to DC-V) of methanolic extracts of aerial parts of Andrographis paniculata (AP) and mature stems of Tinospora cordifolia (TC).

Materials and Methods : The coarse powder (40-mesh) of shade dried aerial parts of AP and mature stems of TC (500g each) was subjected separately tosuccessive extraction with 1000ml each of petroleum ether (60-80oC) followed by methanol. The doses of DC-I to DC-V were selected and were administered orally at doses of 200 mg/kg body weight to Albino Wistar rats and compared with the control group and standard drug i.e. cyclophosphamide (100mg/kg) respectively.

Results: Results suggest that although all the five tested combinations (DC-I to DC-V) prepared using different proportion of methanol extracts of A. paniculata and T. cordifolia (100:Zero, 75:25, 50:50, 25:75 and Zero:100) showed a significant increase in macrophase count, neutrophil adhesion(NA), haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) response. In rats immunized with sheep RBC, DC-I to DC-V enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the footpad thickness response to sheep RBC in sensitized rats on chosen experimental models.

Conclusion: The values of macrophase, NA, HA, DTH and cellular responses of combination DC-II at a 200 mg/kg body weight were statistically significant as compared to other combinations. Combination DC-II exhibited best immunostimulant potential, indicating that it has promising immunomodulatory activity, as proposed from commercial point of view.


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Author Biography

Rajesh Yadav, Department of Pharmacy,
SRMS, College of Engg.and Tech., Bareilly, U.P.

Pharmacy #rd in UPTU, LKO



How to Cite

Yadav R, Yadav N, Kharya MD. . Int J of Pharmc Res [Internet]. 2016Jan.29 [cited 2020May30];6(1):29-40. Available from:


Vol. 6 No. 1 (2016): Jan


Research Articles

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