A Study on in vitro antiviral activities of lyophilized extracts of Glycyrrhiza glabra on Hepatitis B Virus

by
Methodology

Authors


  • Sangeetha Vani


    Ethiraj College For Women,Chennai-8.
    Presidency College,chennai-5.

  • S. Rajarajan


    Vice Chancellor, SRM University, Delhi

DOI:

https://doi.org/10.7439/ijpr.v6i6.3309

Abstract

The present study is to determine the effect of lyophilized extracts of different solvents of Glycyrrhiza glabra on Hepatitis B. The lyophilized plant extracts were collected and studied for its cytotoxicity in HepG2 cell line and in vitro antiviral activity of these extracts was investigated by HBs Ag binding Inhibition Assay, Hepatitis B Virus DNA Polymerase Inhibition Assay using fluorescent probes. The results from Glycyrrhiza glabra were promising in acting as a potent antiviral agent.

Downloads

Download data is not yet available.

Author Biography

Sangeetha Vani, Ethiraj College For Women,Chennai-8.
Presidency College,chennai-5.

Assistant Professor,

Department of Microbiology,

Ethiraj College for Women,

Chennai-8.

References

Mustafa Sunbul, Hepatitis B virus genotypes : Global distribution and clinical importance.World J Gastroenterol 2014 May 14; 20 (18):5427-5434.

Adrian M. Di Bisceglie, M.D. Hepatitis B And Hepatocellular Carcinoma, Hepatology. 2009 May ; 49(5 Suppl): S56–S60.

Palmer Beasley R, Chia-Chin Lin , Lu-Yu Hwang , Chia-Siang Chien ,Hepatocellular Carcinoma and Hepatitis B virus:A Prospective Study of 22 707 Men in Taiwan .The lancet,Volume 318, Issue 8256, 21 November 1981, Pages 1129-1133.

Chan Ran You, Sung Won Lee, Jeong Won Jang, Seung Kew Yoon – Update on hepatitis B Virus infection, World Journal of Gastroenterology 2014 October 7; 20(37):13293-13305.

Fabien Zoulim, David Durantel .Antiviral Therapies and Prospects for a Cure of Chronic Hepatitis B.Cold Spring Harb Perspect Med 2015;5:a201501.

Tao Shen, Xin-Min Yan, Jin-Ping Zhang, Jin-Li Wang, Rong-Xia Zao, Li Li, and Lin-Pin Wang, – Evolution of Hepatitis B Virus in a Chronic HBV-Infected Patient over 2 Years., Hepatitis Research and Treatment volume 2011, Article ID 939148, 6 pages.

Hamid Ullah Wani, Saad Al Kaabi, Manik Sharma, Rajvir Singh, Anil John, Moutaz Derbala and Muneera J. Al-Mohannadi, Clinical Study High dose of Lamivudine and Resistance in Patients with Chronic Hepatitis B, Hindawi Publishing Corporation – Hepatitis Research and Treatment, volume 2014, Article ID 615621, 5 pages.

Thyagarajan SP, Jayaram S, Gopalakrishnan V,Hari R,Jeyakumar P and Sripathi MS . Herbal medicines for liver diseases in India. Journal of Gastroenterology and Hepatology(2002) 17, S370-S376.

Subramoniam A,Pushpangadan P. Development of phytomedicines for liver diseases. Indian Journal of Pharmacology 1999;31: 166-175.

Sanjai Saxena,Glycyrrhiza glabra :Medicine over the Millennium, Natural Product Radiance Vol4(5) September-October 2005.

Usman A Ashfaq, Muhammad S Masoud, Zafar Nawaz, Sheikh Riazuddin, Glycyrrhizin as antiviral agent against Hepatitis C Virus, Journal of Translational Medicine, 2011, 9:112.

Sangeetha Vani G, Rajarajan S, A study on insilico analysis of phytochemicals targeting the proteins of Hepatitis B and C Virus.Int.J.Curr.Microbiol.App.Sci(2015)4(12):683-691.

Jeevan M,Nasreen N,Dinesh S et al.Anti HBV activity of HD-03/ES, a herbal medicine by interference of HBsAg binding to its receptor.Journal off Pharmacy Research 2012,5(8),4348-4352.

Bengt Lofgren, Erik Nordenfelt and Bo Oberg, – Inhibition of RNA – DNA-dependent duck hepatitis B virus DNA polymerase activity by nucleoside and pyrophosphate analogs, Antiviral Research, 12 (1989) 301-310

Antiviral activity of G.glabra on Hepatitis B

Kannan Mohan, Rajendran Paramasivam, Pratap Chandran, Vedha Veerasami, Ashok Gunasekaran, Anushka shanmugam and Karthikeyan Murugesan – Inhibition of Hepatitis B virus DNA polymerase and modulation of TH1 & TH2 Cytokine secretion by three Indian medicinal plants and its correlation with antiviral properties, Journal of Pharmacy Research 2011, 4(4),1044-1046.

Sandeep R.Varma ,Sundaram R,Gopumadhavan,Satyakumar vidyashankar and Pralhad S.Patki.HD-03/ES:A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in transfected Human Hepatocarcinoma PLC/PRF/5 Cells.Hepatitis Research and Treatment .Vol 2013,Article ID 125398,6 pages.

Wing Lam,Ying Li, Jieh-Yuan Liou, Ginger E, Dutschman, and Yung –chi Cheng.Reverse Transcriptase activity of Hepatitis B Virus (HBV) DNA Polymerase within Core Capsid : interaction With Deoxynucleoside triphosphates and Anti-HBV L-Deoxynucleoside Analog Triphosphates.Mol Pharmacol 65:400-406,2004.

Higgs S , Traul D, B, Davis BS, Kamrud KI, Wilcox CL and Beaty BJ, – Fluorometric Assay for DNA Polymerases and Reverse Transcriptase, BioTechniques 21:664-672 (October 1996).

Anderson JP, Bernhard Angerer and Lawrence A, Loeb, Incorporation of reporter-labeled nucleotides by DNA polymerases, BioTechniques 38:257-264 (February 2005)

Silprasit K, Thammaporn R, Tecchasakul S, Hannongbua S, Choowongkomon K.Simple and rapid determination of the enzyme kinetics of HIV-1 reverse transcriptase and anti-HIV-1 agents by a fluorescence based method. J Virol Methods.2011 Feb;171(2):381-7.

Tae Gyun Kim, Seog Youn Kang, Ki Kyung Jung, Ju Hye Kang, Euna Lee, Hyung Mee Han and Seung Hee Kim – Antiviral activities of Extracts Isolated from Terminalis Chebula Retz., Sanguisorba officinalis L., Rubus coreanus Miq. and Rheum palmatum L. against Hepatitis B Virus, Phytother, Res. 15, 718-720 (2001).

Subhashini MS, Rajendran P.Invitro screening of anti HBV and HIV properties of

Gymnema Sylvestre R.Br leaves from Kolli Hills, Tamilnadu, India.Int.J.Curr.Microbiol.App.Sci (2015)4(3):542-547.

  • PDF

     

Published

2016-06-30

How to Cite

1.
Vani S, Rajarajan S. . Int J of Pharmc Res [Internet]. 2016Jun.30 [cited 2020May29];6(6):206-9. Available from: https://ssjournals.net/index.php/ijpr/article/view/3309

Issue

Vol. 6 No. 6 (2016): Jun

Section

Research Articles

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
    4. Author must Submit Copyright form  After acceptance of Article

Dr. Jun Ren is a dedicated and experienced registered dietitian and nutritionist who is committed to helping people achieve their health goals through personalized nutrition plans. With a passion for promoting healthy eating habits and preventing chronic diseases, Dr. Ren has been able to assist numerous clients in improving their overall quality of life.

...

Leave a Comment