DOI:
https://doi.org/10.7439/ijbar.v8i8.4297
Keywords:
Gradient elution, RP-HPLC, Oxybenzone, Octinoxate, Avobenzone
Abstract
Radioprotective mechanisms of Rutin (RUT) and Quercetin (QRT) against gamma radiation was studied by investigating recovery of endogenous spleen colony-forming units, haematological alterations in peripheral blood in Swiss albino mice. Radiation induced haematological alterations in peripheral blood was studied; mice were treated with RUT (10mg/kg.b.wt.) and QRT (20mg/kg.b.wt.) once daily for five consecutive days and exposed to 7.5 Gy of gamma radiation after the last administration. RUT and QRT treatment before exposure to 7.5 Gy of gamma radiation resulted in a significant (p 0.01) increase in cellular components of blood at various post-treatment time points when compared with the respective irradiated groups. Irradiation of mice reduced the spleen colony forming units in the spleen and the number of spleen nodules was inversely related with the increase in radiation dose. The potent antioxidant nature of RUT and QRT mitigate the oxidative stress induced by gamma radiation and thus protect the mice from hemopoietic damage.
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References
. Hall EJ 2000. Acute effects of total-body irradiation, Radiobiology for the Radiologist. Philadelphia, PA: Lippincott Williams & Wilkins.
. Quintiliani M. The oxygen effect in radiation inactivation of DNA and enzymes. Int J Radiat Biol Relat Stud Phys Chem Med. 1986; 50(4):573-94.
. Ross GM. Induction of cell death by radiotherapy. Endocr Relat Cancer 1999; 6(1):41-44.
. Patt HM, Tyree EB, Straube RL, Smith DE 1949. Cysteine Protection against X Irradiation. Science; 110: 213-214.
. Sweeney TR. A survey of compounds from the antiradiation drug development program of the U.S. Army Medical Research Command. . In: Institute WRA, editor. Washington. D. C. 1979; 15-650.
. Arora R, Goel H. Herbal Radioprotectors; Proceedings of Indian Society for Radiation Biology by Regional Cancer Centre (RCC), Thiruvananthapuram, India 2000; 17-19.
. Nair CKK, Parida DK, Nomura T. Radioprotectors in radiotherapy. J Radiat Res (Tokyo); 2001; 42(1):21-37.
. Weiss JF, Landauer MR. Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology 2003; 189(1-2):1-20.
. Maurya DK, Devasagayam TP, Nair CKK. Some novel approaches for radioprotection and the beneficial effect of natural products. Indian Journal of Experimental Biology 2006; 44:93-114.
. Coleman CN, Blakely WF, Fike JR, MacVittie TJ, Metting NF, Mitchell JB. Molecular and cellular biology of moderate-dose (1-10 Gy) radiation and potential mechanisms of radiation protection: report of a workshop at Bethesda, Maryland, December 17-18, 2001. Radiat Res 2003; 159(6):812-34.
. Gulcin I, Mshvildadze V, Gepdiremen A, Elias R. Antioxidant activity of saponins isolated from ivy: alpha-hederin, hederasaponin-C, hederacolchiside-E and hederacolchiside-F. Planta Med 2004; 70(6): 561-563.
. Kostyuk VA, Potapovich AI. Antiradical and chelating effects in flavonoid protection against silica-induced cell injury. Arch Biochem Biophys 1998; 355: 43-48.
. Kong S. E., Blennerhassett, L. R., Heel, K. A., McCauley, R. D., & Hall, J. C. Ischaemia-reperfusion injury to the intestine. Australian and New Zealand Journal of Surgery 1998; 68:554–561.
. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin, and hesperidin on adjuvant arthritis in rat. Farmaco 2001; 56(9): 683-687.
. Bond VP, Fliedner TM, Archambeau JO. Mammalian Radiation Lethality, A Disturbance in Cell Kinetics. Academic Press, New York 1965; pp. 340.
. Milas L, Hunter N, Ito H, Peters LJ. In vivo radioprotective activities of diethyldithiocarbamate (DDC). Int J Radiat Oncol Biol Phys 1984; 10(12): 2335-2343.
. Ijiri K, Potten CS. Radiation-hypersensitive cells in small intestinal crypts; their relationships to clonogenic cells. British Journal of Cancer. 1986 Supplement; 7: 20-22.
. Hall EJ 2000. Acute effects of total-body irradiation, Radiobiology for the Radiologist. Philadelphia, PA: Lippincott Williams & Wilkins.
. Storm HM, Oh SY, Kimler BF, Norton S. Radioprotection of mice by dietary squalene. Lipids 1993; 28(6): 555-9.
. Till JE, MeCulloch EA. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res 1961; 14:213-222.
. Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, CA Cancer J Clin 2002; 55(2): 74-108.
. Little MP. Cancer after exposure to radiation in the course of treatment for benign and malignant disease. Lancet Oncol 2001;
(4): 212-220.
. Coleman CN. Radiation oncology–linking technology and biology in the treatment of cancer. Acta Oncol 2002; 41(1): 6-13.
. Grant S, Dent P. Overview: rational integration of agents directed at novel therapeutic targets into combination chemotherapeutic regimens Curr Opin Investig Drugs 2001; 2(11): 1600-1605.
. Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutat Res 2005; 579(1-2): 200-213.
. Shrikant L. Patil, B. Nageshwar Rao, H. M. Somashekarappa, Rajashekhar K. P. Antigenotoxic potential of rutin and quercetin in swiss mice exposed to gamma radiation. Biomed J 2014; 37:5, 305-313.
. Shrikant L. Patil, H. M. Somashekarappa, Rajashekar Patil. Antioxidative and radioprotective potential of rutin and quercetin in Swiss albino mice exposed to gamma radiation. Journal of Medical Physics 2013; 38:2, 87-92.
. Shrikant L. Patil, H. M. Somashekarappa, Rajashekar Patil. Radiomodulatory role of Rutin and Quercetin in Swiss Albino mice exposed to the whole body gamma radiation. Indian J. of Nucl Med 2012; 27: 237-42.
. Shrikant L. Patil, H. M. Somashekarappa, Rajashekar Patil. Evaluation of the radioprotective action of rutin in mice exposed to gamma-radiation. International Journal of Biological &
Pharmaceutical Research 2012; 3 (1): 12-18.
. Shrikant L. Patil, H. M. Somashekarappa, Rajashekar Patil. Ameliorative Effect of Rutin against Oxidative Stress in Mice Induced By Gamma-Irradiation. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2011; 2 (4): 694-701.
. Tubiana M, Carde P, Frindel E. Ways of minimising hematopoietic damage induced by radiation and cytostatic drugs–the possible role of inhibitors. Radiother Oncol 1993; 29: 1-17.
. Dainiak N, Waselenko JK, Armitage JO, MacVittie TJ, Farese AM. The hematologist and radiation casualties. Hematology 2003; 473-496.
. Maezawa H, Ohizumi Y, Tamai Y, Fukuhara N, Ando F, Kann Y, Tsuda M, Mori T. Survival of mice and hematopoietic stem cells in bone marrow after intermittent total body irradiation. Radiat Med 1987; 5(6): 215-219.
. Wu AM, Till JE, Siminovitch L, McCulloch EA. A cytological study of the capacity for differentiation of normal hemopoietic colony-forming cells. J Cell Physiol 1967; 69: 177-184.
. Becker AJ, McCulloch EA, Till JE. Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature 1963; 197:452-454.
. Siminovitch L, McCulloch EA, Till JE. The distribution of colony forming cells among spleen colonies. J. Cell Physiol 1963; 62: 327-336.
. Zhou Y, Mi MT. Genistein stimulates hematopoiesis and increases survival in irradiated mice. J Radiat Res (Tokyo) 2005; 46(4):425-433.
. Miyanomae T, Frindel E. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol 1988; 16(9): 801-806.
. Ganasoundari A, Zare SM, Devi PU. Modification of bone marrow radiosensensitivity by medicinal plant extracts. Br J Radiol 1997; 70(834):599-602.
. Goel HC, Prasad J, Singh S, Sagar RK, Kumar IP, Sinha AK. Radioprotection by a herbal preparation of Hippophae rhamnoides, RH-3, against whole body lethal irradiation in mice. Phytomedicine 2002; 9(1): 15-25.
. Goel HC, Prasad J, Singh S, Sagar RK, Agrawala PK, Bala M, Sinha AK, Dogra R. Radioprotective potential of an herbal extract of Tinospora cordifolia. J Radiat Res (Tokyo); 2004; 45(1): 61-8.
. Hoat J, Barkina NF. Quantitative study of haemopoietic recovery after a sublethal X-irradiation in the mouse. Acta Haematol 1969; 42: 347.
. Goldin EM, Neff RD. Lymphocyte depletion in peripheral blood of acutely gamma-irradiated rats. Int J Radiat Biol Relat Stud Phys Chem Med 1975; 27(4): 337-342.
. Daga SS, Jain VK, Goyal PK. Radioresponse of Leucocytes in Peripheral Blood of Mice against Gamma Irradiation and their Protection by Liv.52. Probe 3 1995; 222.
. Uma Devi P. Normal tissue protection in cancer therapy–progress and prospects. Acta Oncologica 1998; 37: 247-252.
. Potten CS, Morris RJ. Epithelial stem cells in vivo. Journal of Cell Science. Supplement 1988; 10:45-62.
. Potten CS. A comprehensive study of the radiobiological response of the murine (BDF1) small intestine. Int J Radiat Biol 1990; 58(6): 925-973.
. Indran M, Carr KE, Gilmore RS, Boyle FC. Mucosal changes in mouse duodenum after gamma-irradiation or reserpine treatment. J Submicrosc Cytol Pathol 1991; 23(2): 267-278.
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