Abstract
A simple precise reproducible U.V. Spectrophotometric methods have been developed and validated for the simultaneous estimation of RPS and LSP in capsule dosage form. This Paper describes 2 methods for the simultaneous estimation of both the drugs. The simultaneous equation method (SEM) which involves the measurement of absorbance of RPS and LSP at 231.8 nm and 284.0 nm respectively and the absorbance ratio method (ARM) which involves the measurement of absorbance of the mixture of both the drugs at 259.0 nm which is isobestic point and at 284 nm ƛ max of RPS respectively. The developed method obeys the beers law in the concentration range of 2-10µg/ml for RPS and 7.5-37.5 µg/ml for LSP. The recovery studies shows %RSD for LSP 1.58, 0.52, 0.45 and for RPS 1.79, 1.52, 0.63 by ARM method and the recovery studies shows %RSD for LSP 0.23, 0.53, 0.88 and for RPS 0.76, 1.71, 1.56 by SEM method. The results of analysis have been validated statistically for accuracy, Precision, Repeatability, Specificity, and Ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form.
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DOI: http://dx.doi.org/10.7439/ijpc.v3i4.59
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Copyright (c) 2014 International Journal of Pharmaceutical Chemistry
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